Figure 2. Visual analysis of cell-culture derived WNVL1 strain NY397-99.

Flavivirus gene-specific PCR amplicons were identified using oligonucleotide microarray probes grouped by virus genus and gene, (A) Flavivirus E gene, (B) Flavivirus NS3 gene, and (C) Flavivirus NS5 gene, and sorted into subgroups based on phylogenetic clade and target virus(es). ECD signals were converted into z-scores. Subgroups with average z-scores greater than 10 were considered positive and used for virus identification. The plotted maximum z-scores represent the greatest individual probe z-score within a subgroup and were used to determine cross-hybridization. The PCR amplicons hybridized with probes in the Flavivirus generic, JEV clade, WNVL1/KUNV, and WNVL1 subgroups for all three gene targets. WNVL1 was differentiated from KUNV by the less than 10 z-scores for the KUNV subgroups. The virus abbreviations are defined in Table S1.