Figure 5. Visual analysis of cell-culture derived BUNV strain 131B-06.

Orthobunyavirus gene-specific PCR amplicons were identified using oligonucleotide microarray probes grouped by virus genus and segment, (A) Orthobunyavirus S segment, (B) Orthobunyavirus M segment, and (C) Orthobunyavirus L segment, and sorted into subgroups based on phylogenetic clade and target virus(es). ECD signals were converted into z-scores. Subgroups with average z-scores greater than 10 were considered positive and used for virus identification. The plotted maximum z-scores represent the greatest individual probe z-score within a subgroup and were used to determine cross-hybridization. The PCR amplicons hybridized with probes in the BUNV subgroups for the M and L segment targets but virus-specific probes could not be designed for the S segment. The BUNV amplicons did have z-scores greater than 10 for seven of the 42 S segment BUNV clade probes, but the average subgroup z-score was less than 10. The virus abbreviations are defined in Table S1.