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. 2013 Aug 15;7(8):e2349. doi: 10.1371/journal.pntd.0002349

Table 4. PCR amplification and microarray detection of alphaviruses.

PCR amplification/microarray detectiona
Virus Strain nsP1 nsP4 E1 Mosquito speciesb
Aura RIID 1990 POS/yes POS/yes POS/yes n.t.
Babanki Ken07-46A-49 POS/yesf POS/yesf POS/yesf n.t.
Chikungunya INDO23574 POS/yes POS/yes POS/yes Ae. taeniorhynchus
Getah ROK-2.0017 POS/yes POS/yes POSc/yes n.t.
Mayaro TR467 POS/yes POS/yes POS/yes n.t.
Ndumu Ken07-332 POS/yesd e POS/yes NEGd n.t.
Ockelbo ISL-44 POS/yesf POS/yesf POS/yesf n.t.
O'nyong nyong Gulu POS/yesg POS/yesg POS/yesg n.t.
Ross River T-49 POSc/yes POSc/yes NEG Ae. albopictus
Semliki Fortest Ken07-586 POS/yes POS/yes POS/yes n.t.
Sindbis Ken07-611 POS/yesf POS/yesf POS/yesf Ae. aegypti
Una PE-1.0800 POS/nod POS/yes POS/nod n.t.
VEE TC83 POS/yes POS/yes POS/yes n.t.
WEE McMillan POS/yes POS/yes POS/no Ae. albopictus

Viral RNA derived from cell culture or infected mosquitoes were PCR amplified using GSPs. Amplicons were analyzed using the ArboChip5.1 microarray. VEE, Venezuelan equine encephalitis; WEE, western equine encephalomyelitis; POS, positive PCR amplification; NEG, negative PCR amplification; yes, detected by microarray; no, not detected by microarray; n.t., not tested.

a

Viruses propagated in cell culture and identified by microarray to species unless otherwise noted.

b

Virus-infected mosquito used for microarray evaluations, microarray detected at least one target for each virus.

c

PCR amplification produced a weak visible band.

d

ArboChip5.1 does not include probes specific to the target.

e

Microarray detection with genus-level probes only.

f

Target was detected but could not be differentiated between BBKV, OCKV, and SINV.

g

Target was detected but could not be differentiated between ONNV and IOV.