Skip to main content
. 2013 Aug 15;7(8):e2349. doi: 10.1371/journal.pntd.0002349

Table 5. PCR amplification and microarray detection of phleboviruses.

PCR amplification/microarray detectiona
Virus Strain S M L Mosquito speciesb
Rift Valley fever ZH501 POS/yes POS/yes POSe/yes Cx. pipiens
Rift Valley fever ZH548 POS/yes POS/yes POSe/yes n.t.
Sandfly fever Naples 85-055 POS/yes POS/noc POS/yesc d n.t.
Toscana ISS PHI3 POS/yes POS/yes POS/yes n.t.
Candiru BeH2251 POS/yes POS/yes POS/yes n.t.
Chagrese LW10 POS/noc POS/noc POS/yesc d n.t.

Viral RNA derived from cell culture or infected mosquitoes were PCR amplified using GSPs. Amplicons were analyzed using the ArboChip5.1 microarray. POS, positive PCR amplification; NEG, negative PCR amplification; yes, detected by microarray; no, not detected by microarray; n.t., not tested.

a

Viruses propagated in cell culture and identified by microarray to species unless otherwise noted.

b

Virus-infected mosquito used for microarray evaluations, microarray detected at least one target for each virus.

c

ArboChip5.1 does not include probes specific to the target.

d

Microarray detection with genus-level probes only.

e

Not a target virus on ArboChip5.1.