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| Lower limits of detection (PFUe) | |||
| Virus | qPCR | Conv. PCR | Microarray |
| West Nile | 200 | 200 | 200 |
| Sindbis | 10 | 10 | 100 |
| Rift Valley fever | 125 | 125 | 1,250 |
The lower limits of detection determined for infected mosquitoes were evaluated by using 10-fold serial dilutions of RNA extracted from one infected mosquito pooled with 24 uninfected mosquitoes. PFUe, plaque forming unit equivalents; qPCR, real-time PCR, Conv. PCR, conventional PCR.
