Table 8. Microarray identification of flavivirus RNA from Thailand mosquitoes (2011–2012).
| PCR result/microarray identification | |||||
| Pool No.a | Mosquito species (pool size) | E | NS3 | NS5 | Sequencing resultb |
| Th9-0011 | Cx. quinquefasciatus (1) | NEG | NEG | POS/CxFV | CxFV |
| Th9-0024 | Cx. vishnui (2) | POS/n.d. | NEG | NEG | QBV |
| Th9-0032 | Cx. quinquefasciatus (6) | POS/n.d. | POSc/n.d. | POSc/QBV | QBV-liked |
| Th9-0122 | Ae. aegypti (1) | POS/DENV3 | POS/DENV3 | POS/DENV3 | DENV3 |
| Th9-0164 | Cx. tritaeniorhynchus (25) | POS/n.d. | POS/JEV | POS/JEV | JEV |
| Th9-0175 | Cx. tritaeniorhynchus (25) | POS/n.d. | POS/JEV | POS/JEV | JEV |
| Th10-0040 | Cx. vishnui (20) | NEG | POS/TMUV | POS/n.d. | TMUV |
| Th10-0121 | Cx. vishnui (20) | NEG | POS/TMUV | POS/TMUV | TMUV |
| Th10-0217 | Cx. vishnui (20) | NEG | POS/TMUV | POS/TMUV | TMUV |
| Th10-0552 | Cx. vishnui (20) | NEG | POS/TMUV | POS/TMUV | TMUV |
| Th10-0578 | Cx. fuscocephala (16) | NEG | POS/n.d. | NEG | WTVe |
| Th10-0591 | Cx. gelidus (20) | NEG | NEG | POSc/n.d. | QBV |
| Th10-0767 | Cx. gelidus (2) | NEG | NEG | POSc/n.d. | QBV |
cDNAs from mosquito pools that were found to contain viral RNA by flavivirus screening methods were PCR amplified using GSPs and analyzed using the ArboChip5.1 microarray for virus identification. POS, positive PCR amplification; NEG, negative PCR amplification; n.d., no detection; CxFV, Culex flavivirus; QBV, Quang Binh virus; DENV3, dengue virus type 3; JEV, Japanese encephalitis virus; TMUV, tembusu virus; WTV, Wang Thong virus.
a
Mosquito pool No. beginning with Th9 were collected in 2011, Th10 were collected in 2012.
b
Identifcations based on greater than 90% identity using partial NS5 sequences.
c
PCR amplification produces a weak visible band.
d
Partial NS5 sequence 78.3% identical to QBV.
e
WTV targeted probes not included on ArboChip5.1.