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. 2013 Aug 15;9(8):e1003525. doi: 10.1371/journal.ppat.1003525

Figure 5. Confirmation of the role of SCP by structural comparison of SCP-deficient and wild-type viral particles.

Figure 5

(A) Representative cryoEM images of SCP-deficient viral particles showing enveloped particles without the dsDNA genome. (B, C) Radially colored surface representations of 3D reconstructions of wild type (B) and SCP-deficient (C) HCMV viral particles at 20 Å resolution. Lower panels are zoom-in views of the region containing a triplex, revealing that pp150 is present in the wild-type structure but absent in the SCP-deficient viral particles. (D, E) 15 Å-thick central slices extracted from reconstructions of wild type (D) and SCP-deficient (E) particles respectively. Concentric shells of density inside the capsid in (D) are attributable to the viral dsDNA genome, and they are uniformly spaced (23 Å). A ring of scaffold densities are identified in (E), but there is no DNA density. Small bulge on tip of MCP in (D) corresponds to the density of SCP. There is no such bulge at the corresponding position in the SCP-deficient reconstruction.