Figure 7. NMR analysis of outer spore walls in the paralog mutant strains.

A) The dityrosine region of the ¹³C NMR spectrum is shown for spore walls from wild type, dit1Δ and the indicated paralog mutant strains. Positions of the dityrosine chemical shifts and chitin carbonyl chemical shifts are indicated above the wild type spectrum. The spectra have been scaled so the height of the carbonyl resonance is constant. B) The component χ region of the ¹³C NMR spectrum is shown for spore walls from wild type, dit1Δ and the indicated paralog mutant strains. Positions of the component χ chemical shifts and chitin methyl group shifts are indicated above the wild type spectrum. The spectra have been scaled to the C5/3 resonance as in Figure 6. Multiple mutant diploids are indicated by the name of the set of genes deleted as listed in Table 2.