Figure 5. Spo11 and Ubc5 are required to complete sporulation during unisexual reproduction.

Wild type XL280α and spo11Δ and ubc5Δ mutants were incubated on V8 medium in the dark at room temperature for 14 days. (A) Hyphal development was evaluated by light microscopy. The edges of the colony produced abundant hyphae in WT, spo11Δ, and ubc5Δ mutants (upper row). In XL280α, basidia were decorated with four spore chains, whereas in spo11Δ and ubc5Δ mutants, spore production was severely impaired and naked basidia or at most one or two short spore chains were observed. The scale bars represent 100 µm for the upper row and 10 µm for the middle rows. (B) Scanning electron microscopic analysis of sporulation defects during unisexual reproduction. The scale bars represent 5 µm.