Table 4. X-irradiation-induced DSBs partially rescue the spore survival defect of spo11Δ mutants during bisexual and unisexual reproduction.
| X-ray dose (kRad) | Viable cells/mla | Unisexual reproduction progeny viability | Bisexual reproduction progeny viability | Total progeny | ||||
| XL280α (%) | spo11Δ (%) | ubc5Δ (%) | JEC21α×JEC20a (%) | α spo11Δ×spo11Δ a (%) | α ubc5Δ×ubc5Δ a (%) | |||
| 0 | 5.3×108 | 23 (76%) | 1 (3%) | 2 (6%) | 25 (83%) | 2 (6%) | 4 (13%) | 30 |
| 1 | 5.4×108 | 20 (66%) | 0 (0%) | 1 (3%) | 28 (93%) | 3 (10%) | 3 (10%) | 30 |
| 3 | 5.1×108 | 21 (70%) | 0 (0%) | 3 (10%) | 26 (86%) | 0 (0%) | 4 (13%) | 30 |
| 5 | 4.8×108 | 22 (73%) | 2 (6%) | 2 (6%) | 24 (80%) | 1 (3%) | 5 (16%) | 30 |
| 10 | 4.4×108 | 18 (60%) | 4 (13%) | 0 (0%) | 22 (73%) | 5 (16%) | 2 (6%) | 30 |
| 20 | 3.1×108 | 16 (53%) | 7 (23%) | 2 (6%) | 20 (66%) | 8 (26%) | 3 (10%) | 30 |
Cultures were incubated on V8 medium for six days for unisexual reproduction and four days for bisexual reproduction. Plates were irradiated at the designated dose and incubated in the dark for two days to allow spore production. A total of 30 spores were isolated from each culture.
a
Viable CFU determined from vegetative growth on solid media independently for XL280α, spo11Δ, ubc5Δ, and JEC21α. The viable CFU for each strain at the same dose was similar with minor differences. Here we present the viability of XL280α as reference.