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. 2013 Aug 15;8(8):e72650. doi: 10.1371/journal.pone.0072650

Figure 3. Enforced HIF-1α expression in RASF upregulated IL-33 levels.

Figure 3

A, RASF were transfected with pcDNA3.1 or pcDNA3.1-HIF-1α for 24 h, then were stimulated with 10 ng/ml TNF-α plus 2 ng/ml IL-1β. The HIF-1α mRNA levels (A) as well as protein levels (B) were first assayed to confirm the overexpression. Cells were harvested 4 h after stimulation for realtime PCR (C) and RT-PCR (D) analyses of IL-33. 24 h later, cells were collected for measuring IL-33 protein levels (E) by cell lysate ELISA (Wilcoxon signed-rank test, *p<0.05, **p<0.001). The results were presented as mean±SEM of four independent experiments.