Figure 7. Genetic modulation of SMN-dependent proliferation in NIH3T3 cells.

Dose-response analysis of lentiviral-mediated human SMN expression on cell proliferation of SMN-deficient NIH3T3-Smn_RNAi and NIH3T3-SMN2_low/Smn_RNAi cells. In these experiments, NIH3T3 cells were cultured for 5 days with Dox prior to seeding in six replicate wells of a 96-well plate in the presence of Dox. Vehicle or increasing amounts of SMN-expressing lentivirus were added 4 hours later. For each group and treatment, cell number was determined at 5 days post-plating and normalized to that of the corresponding vehicle-treated cells. Data are represented as mean and SEM (n = 6; * = p<0.05; ** = p<0.01; *** = p<0.001; one-way ANOVA).