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. 2013 Aug 15;8(8):e72118. doi: 10.1371/journal.pone.0072118

Figure 4. Cellular localization of BODIPY-DIF-3 in HeLa cells.

Figure 4

(A) Cells were incubated for 0.5 h with BODIPY-DIF-3 (20 µM), Hoechst (0.1 µg/ml) and MitoTracker (0.1 µM), washed free of the additives, and observed by using a high-magnification fluorescence microscope. (B–D) Cells were incubated for 1–3 days with BODIPY-DIF-3 (20 µM) and then for 0.5 h with Hoechst (0.1 µg/ml) and MitoTracker (0.1 µM). Cells were washed free of the additives and observed by using a high-magnification fluorescence microscope. Three-dimensional (3D) images were constructed from z-stacked 2D images, and two representative 2D-projections of the 3D images are shown (A–D). BODIPY-DIF-3 and MitoTracker co-localized to the mitochondria.