Table 1. Comparison of the effects of DIF-like molecules and CCCP.
| IC50 (µM) vs. Cell growth | PC100 (µM) for | |||||
| Compound | M.W. | HeLa | LM8 | 3T3-L1 | Mito. O2 consumption | ClogP |
| DIF-3 | 272.7 | 14.5 | 15.5 | >20 | 12 | 3.79 |
| Bu-DIF-3 | 314.8 | 3.2 | 2.0 | 4.3 | 1.7 | 5.38 |
| BODIPY-DIF-3 | 603.9 | 13.2 | 12.2 | 20 | 25 | 7.02 |
| Bu-BODIPY | 347.2 | >20 | >20 | >20 | >100 | 4.45 |
| CCCP | 204.6 | 5.2 | 6.8 | 7.5 | 0.43 | 3.40 |
Footnotes. IC50 (50% inhibitory concentration) values versus cell growth of HeLa, LM8, and 3T3-L1 cells were determined from drug dose–dependent growth assays. The PC100 (100% promoting concentration) values for the control rate of mitochondrial (Mito.) O2 consumption were calculated from data in Figure 8 as described in the Methods section. The hydrophobic index (ClogP) for each compound was calculated by using the ChemDraw10.0 software (Cambridge Soft, UK) to allow deduction of the membrane permeability of each compound.