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. 2013 Aug 15;8(8):e72121. doi: 10.1371/journal.pone.0072121

Figure 2. Inhibition of MMP-9 enhances satellite cell population in skeletal muscle of mdx mice.

Figure 2

(A) Representative photomicrograph of TA muscle section from 8-week old mdx;Mmp9+/+ and mdx;Mmp9+/− stained for Pax7 and DAPI. Arrows point to Pax7+ satellite cells. Scale bar: 50 µm. (B) Quantification of relative number of Pax7+ cells per myofiber in TA muscle sections from 8-week old mdx;Mmp9+/+ and mdx;Mmp9+/− mice. (C) Representative FACS dot plots showing the percentage of satellite cells in GA muscle of 8-week old mdx;Mmp9+/+ and mdx;Mmp9+/− mice. Negative selection antibodies (CD45, CD31, Ter119) are in upper box whereas positive selection antibody (α7-integrin) is in lower box. (D) Quantification of satellite cells by FACS in GA muscles of 8-week mdx;Mmp9+/+ and mdx;Mmp9+/− mice. N = 4 in each group Error bars represent SD. *p<0.05 values significantly different from that of mdx;Mmp9+/+ mice.