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. 2013 Aug 15;8(8):e72900. doi: 10.1371/journal.pone.0072900

Figure 6. Expression of CD95 and CD95L protein in RCECs.

Figure 6

A: western blotting analysis showing the presence of CD95 and CD95L protein in RCECs. B, C: Results of statistical analysis of protein levels relative to GAPDH. RCECs cultured in NOP (normal osmotic pressure, 312mOsM) (1), HOP (high osmotic pressure, 500mOsM) (2), HOP+NAC (10 mM) (3), HOP+ DPI (10 µM) (4), HOP+ JNK inhibitor SP600125 (25 µM) (5) for 24 h. CD95 and CD95L expression in the HOP group was significantly higher than that in NOP group (P<0.01). NAC, DPI and JNK inhibitor SP600125 downregulated CD95 expression in RCECs exposed to HOP. NAC and JNK inhibitor SP600125 upregulated CD95L expression in RCECs exposed to HOP, while CD95L expression was decreased significantly in the HOP group added with DPI (P<0.01). (mean±SD, n = 3). ** P<0.01 vs. NOP, ## P<0.01 vs. HOP.