Table 1. Surface phenotype of non-adherent DCreg cells co-cultured with lung stroma for 7 days.*.
| DC surface marker | The source of “educating” lung stromal cells |
|||
|---|---|---|---|---|
| I/St naive | I/St infected | B6 naive | B6 infected | |
| CD11b | 95.6 ± 3.6 | 91.6 ± 2.9 | 91.6 ± 3.9 | 95.3 ± 4.0 |
| CD11c | 9.8 ± 1.1 | 6.3 ± 1.6 | 7.8 ± 2.0 | 5.0 ± 1.9 |
| Gr1 | 45.1 ± 5.0 | 58.7 ± 3.7 | 43.5 ± 4.4 | 54.4 ± 3.9 |
| MHC Class IIhigh | < 5 | < 5 | < 5 | < 5 |
| CD80 | 20.2 ± 2.1 | 18.7 ± 1.8 | 21.0 ± 1.7 | 22.1 ± 2.5 |
| CD103 | < 5 | < 5 | < 5 | < 5 |
* Data are presented as the per cent of cells ± SD. For each experiment a mixture of bone marrow cells from 15–17 mice of each group (~3 x 108) served as a source of Lin- DC precursors (~2% = 4 x 106). Phenotypes were assessed in 3-4 independent experiments and the results summarized. No statistically significant differences between groups were observed, except that Gr1-positive cells comprised significantly (P < 0.05, ANOVA) larger population when DC precursors were co-cultured with stroma from infected compared to naïve mice of both strains (see text).