Figure 11. Diversity and richness of fecal Microbiota in sham and ligated A/J and B6 mice (n = 5 mice for each of four groups). (A) Sobs analysis of number of observed OTUs in A/J and B6 mice following Sham and CBDL procedures. Note that the number of fecal OTUs increases significantly in the B6 mice following CBDL as compared with shams, p < 0.05. There is no significant difference in the number of fecal OTUs in the A/J mice following CBDL as compared with shams. (B) Shannon Diversity Index Analysis of A/J and B6 mice following sham and CBDL procedures. The B6 mice significantly increased their fecal bacterial diversity following CBDL compared with shams, p < 0.05. No significant difference was found in the A/J mice. (C) Chao1 richness estimate analysis of A/J and B6 mice following sham and CBDL procedures. B6 mice had a significantly elevated richness estimate of their fecal bacteria following CBDL compared with shams, p < 0.05. No significant difference was found in the A/J mice.