Figure 1. CTA095 inhibits Etk activity and PC3 cell growth.

Chemical structure of CTA095 (A), identification of CTA095 as a potent Etk inhibitor (B) and its cytotoxicity to PC3 cells (C). For Etk inhibition, purified Etk (20 nM), the corresponding compounds (1 μM), and the peptide substrate (YIYGSFK) were incubated with ³³P-ATP in a kinase reaction. The resulting product was analyzed on a TLC plate. For growth inhibition, PC3 cells were seeded at 5,000 cells/well in 96-well plate overnight and treated with the corresponding compounds (10 μM) The cell viability was measured using MTT assay after 72 h. Columns, mean; bars, standard deviation, n = 3.