Table 2. Comparison of mean values of metabolites consumed or produced in culture media between embryos with or without evidences of morphological changes after 24 hours of individual culture.
| Metabolite | Initial stage | No morphological changes | Morphological changes | p |
| Pyruvate | 2–4 cells | 3.80±0.52 (41) | 6.19±0.66 (47) | 0.0062 |
| uptake | 5–8 cells | 4.26±0.54 (42) | 6.97±0.80 (32) | 0.0048 |
| (pmol/emb/h) | Morula | 6.99±0.98 (17) | 12.57±1.15 (14) | 0.0009 |
| Early blastocysts | 11.53±1.47 (19) | 19.09±1.61 (35) | 0.0031 | |
| Expanded blastocysts | 19.26±1.26 (22) | 24.47±1.41 (25) | 0.0092 | |
| Glucose | 2–4 cells | 2.76±0.44 (35) | 2.38±0.49 (28) | 0.5660 |
| uptake | 5–8 cells | 2.62±0.61 (23) | 2.52±0.52 (18) | 0.9035 |
| (pmol/emb/h) | Morula | 6.29±1.70 (20) | 8.71±1.93 (12) | 0.3703 |
| Early blastocysts | 9.54±1.36 (19) | 16.70±1.43 (38) | 0.0023 | |
| Expanded blastocysts | 25.60±2.05 (37) | 40.88±2.42 (48) | <0.0001 | |
| Lactate | 2–4 cells | 1.24±0.32 (50) | 0.94±0.22 (47) | 0.4514 |
| production | 5–8 cells | 4.36±0.70 (39) | 3.60±0.67 (50) | 0.4419 |
| (pmol/emb/h) | Morula | 12.28±1.53 (26) | 18.24±1.64 (18) | 0.0128 |
| Early blastocysts | 16.81±1.40 (24) | 26.90±1.80 (50) | 0.0005 | |
| Expanded blastocysts | 38.21±3.00 (48) | 59.39±3.06 (75) | <0.0001 | |
| Amino acid | 2–4 cells | 10.38±0.57 (25) | 10.62±0.55 (23) | 0.7630 |
| turnover | 5–8 cells | 12.71±0.69 (21) | 11.50±0.64 (31) | 0.2152 |
| (pmol/emb/h) | Morula | 17.25±0.92 (30) | 24.67±1.44 (18) | <0.0001 |
| Early blastocysts | 23.19±1.34 (29) | 35.29±3.21 (21) | 0.0003 | |
| Expanded blastocysts | 47.50±2.64 (36) | 64.28±3.71 (34) | 0.0004 |
Number of samples analysed are indicated in brackets.
Amino acid turnover is the sum of consumption and production.