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. 2013 Aug 15;8(8):e72347. doi: 10.1371/journal.pone.0072347

Figure 1. Fourth i.c.v. PACAP 6-38 blocks CARTp-induced reductions in solid food intake.

Figure 1

Male Sprague-Dawley rats (n = 8) were pretreated with varying doses of PACAP fragment 6-38 (range 0.3–3 nmol) or vehicle prior to dosing with 0.3 nmol CARTp or vehicle. (A) Solid food intake measures (mean g ±SE) from the entire 22 h observation period. Cumulative solid food intake was recorded at 2 h (B), 5 h (C), and 22 h (D) following peptide injections and food presentation. CARTp significantly inhibited food intake 2–22 h after injection (Fig. 1A–D). Pretreatment with 3 nmol PACAP 6-38 completely blocked the effect after 5 h (Fig. 1 C). Significances from Tukey’s post hoc test after repeated measures ANOVA are indicated above: *p<0.05; **p<0.01; ***p<0.001. The treatment abbreviations are V = saline vehicle; C = 0.3 nmol CARTp; P0.3 = 0.3 nmol PACAP 6-38; P0.6 = 0.6 nmol PACAP 6-38; P3 = 3 nmol PACAP 6-38.