Figure 3. Analysis of the mRNAs whose polysome association was increased after acetic acid treatment.
Overlap of mRNAs with (A) increased and (B) decreased association with polysomes at 15 min or 30 min of acetic acid treatment when compared with control (0 min). (C) Summary of GO terms of the mRNAs altered in the yeast cells upon 15 and 30 min of acetic acid treatment. Heatmaps and clustering of genes were carried out using MeV software [32]. Each colour square represents the average expression level of the genes annotated with the corresponding GO term for each condition. (D) Functional analysis of the mRNAs altered in the microarray analysis of yeast cells upon 15 or 30 min of acetic acid treatment was performed using the Expander software TANGO tool (Algorithms in Computational Genomics group, Blavatnik School of Computer Science, Tel Aviv University, Tel Aviv, Israel) [33] and the Saccharomyces Genome Database GO Term Mapper (db.yeastgenome.org/cgi-bin/SGD/GO/goTermMapper).