Table 1. List of primers for quantification of mRNA expression by qPCR and their efficiency (Eff).
Gene | Primer sequences | Eff (%) |
HSC82 | F: CCGGTGAATCTCTAAAGGCA | 96.3 |
R: ATCAATTGGGTCGGTCAAGA | ||
HSP82 | F: GAGTTGACGAAGGTGGTGCT | 97.9 |
R: ATGCAAAGGAAGTTGGTTCG | ||
TIF4632 | F: TCCGAGGAGACATTAGAGTCCG | 99.1 |
R: ACCGAACCTTCAAGAGTTGCC | ||
CLN3 | F: GCCCCTTTGGAAGCTTTCATT | 98.4 |
R: TGGCACCCAATTTGATCTCG | ||
ACT1 | F: GATCATTGCTCCTCCAGAA | 98.2 |
R: ACTTGTGGTGAACGATAGAT | ||
TDH2 | F: CCGCTGAAGGTAAGTTGA | 95.1 |
R: CGAAGATGGAAGAGTTAGAGT | ||
PDA1 | F: TGACGAACAAGTTGAATTAGC | 94.8 |
R: TCTTAGGGTTGGAGTTTCTG |