Figure 4. Phosphorylation of Erk and CREB by LAD2 cells in response to LTD₄ and LTE₄ and the role of PKC in mediating these effects.

(A) phosphorylation as well as total expression of Erk and CREB proteins by Western blotting in cell lysates of LAD2 cells pre-treated with GFX (2 µM) and stimulated with 500 nM of LTD₄ and LTE₄ respectively for 30 minutes (B) Quantitative analysis. The shown data represents±SD of three separate experiments. *P<0.05. NS = non-significant.