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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Diagn Mol Pathol. 2013 Sep;22(3):149–155. doi: 10.1097/PDM.0b013e31828308a1

Fig. 1.

Fig. 1

Design for TD-PCR. In standard PCR for detecting ITDs, template-specific PCR products are amplified by forward primer F and reverse primer R from both wild-type (top) and mutant (bottom) alleles with preferential amplification of the smaller sized wild-type allele. In the TD-PCR, template-specific PCR products are only amplified from the mutant allele by forward primer F1 and reverse external and internal primers R1 and R2 . “△” indicates the defined size difference of the paired amplicons (19+1 bases in this study). “→” indicates primers.