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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Mitochondrion. 2013 Feb 21;13(6):10.1016/j.mito.2013.02.002. doi: 10.1016/j.mito.2013.02.002

Fig. 3. Identification of mitochondrial biotin carboxylases as SIR-2.2 interacting factors.

Fig. 3

(A) Schematic overview of the strategy used to identify interaction partners of SIR-2.2. A stable integrated transgenic worm strain expressing SIR-2.2 with a C-terminal Strep-GFP-tag under control of the endogenous promoter was generated and used for extract preparation. GFP-tagged SIR-2.2 was immunoprecipitated with anti-GFP-specific antibodies or the anti-SIR-2.2-specific antibodies from total or mitochondrial worm extracts. Isolated proteins were subjected to SDS-PAGE and analyzed by mass spectrometry. (B) Protein sequence of C.elegans D2023.2 (PYC-1). (C) Protein sequence of C. elegans F27D9.5 (PCCA-1). (D) Protein sequence of F32B6.2 (MCCC-1). Peptides identified by mass spectrometric analyses are highlighted on the protein sequence. Total sequence coverage is indicated.