Table 2.
Protein | kcat, s−1 | Km, μM | KDNA, nM |
---|---|---|---|
Wild type | 35 | 110 | 200 |
F64A | 14 | 230 | 380 |
F192A | 42 | 220 | 1,180 |
Y257A | 5 | 310 | 930 |
H587A | 47 | 200 | 440 |
F626A | 18 | 70 | 450 |
Terms are defined in Materials and Methods.
Protein | kcat, s−1 | Km, μM | KDNA, nM |
---|---|---|---|
Wild type | 35 | 110 | 200 |
F64A | 14 | 230 | 380 |
F192A | 42 | 220 | 1,180 |
Y257A | 5 | 310 | 930 |
H587A | 47 | 200 | 440 |
F626A | 18 | 70 | 450 |
Terms are defined in Materials and Methods.