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SV40 Tag immunodominance hierarchy in TRAMP mice primed by DNA vaccination. TRAMP mice were immunized with (A) pΔTag, (B) pDOM-epitope encoding the individual Tag-derived determinants as indicated, (C) pDOM-T5A3T1 or (D) pDOM-T5A3T4. After 12 days, the number of epitope-specific T cells induced was assayed by IFN-γ ELISPOT. The results shown are combined from two separate experiments, with each data point representing an individual mouse. Horizontal bars depict group means.
