Figure 4.

Immunogenicity of epitope T4 kinetic-stability variants and dominance over T5-specific CD8⁺ T-cell responses. Non-transgenic (circles) and TRAMP (squares) mice were immunized with pDOM-T5_A3T4, encoding the T4 MVPs indicated on the top right of each panel. After 12 days, the number of T4 (open) and T5 (closed) epitope-specific CD8⁺ T cells induced were assayed by IFN-γ ELISPOT, using native peptides for the restimulation. The results shown are combined from two separate experiments, with each data point representing an individual mouse.