Figure 6.

Domination of epitope T5-specific CD8⁺ T cells occurs early after activation. (A) Representative structure of pGAL4-T4/CMV-T5 and schema of the modified GeneSwitch system used to temporally regulate T4 expression relative to uncontrolled T5 expression. (B) C57BL/6J mice were immunized with pGAL4-T4/CMV-T5 along with pSwitch, and left untreated or administered with 5 mg/kg MFP at the time points indicated. The number of peptide-specific T cells elicited was determined 12 days later by IFN-γ ELISPOT. The bars indicate group medians and results are representative of two separate experiments. p-Values were determined by the non-parametric Mann–Whitney test (two-tailed).