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. 2013 May 23;199(3):749–757. doi: 10.1111/nph.12304

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Copyright © 2013 New Phytologist Trust

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Fig. 2 — The accumulation levels of Bamboo mosaic virus (BaMV) RNA in NbGSTU4-knockdown protoplasts (a) Northern blot analysis of BaMV RNA in NbGSTU4-knockdown and control (Luc-knockdown) N. benthamiana protoplasts inoculated with BaMV RNA 48 h post-inoculation. rRNA indicates the loading control. (b) The accumulation levels of the plus- and minus-strand BaMV RNA in NbGSTU4-knockdown N. benthamiana protoplasts was measured by real-time RT-PCR. (c) The expression levels of NbGSTU4 in control and NbGSTU4-knockdown N. benthamiana protoplasts were measured by real-time RT-PCR. Control plants, black bars; NbGSTU4, grey bars. The numbers shown above the statistic bar were the average ± SE of at least three independent experiments. Asterisks indicate statistically significant differences between the indicated group analyzed by Student's t-test (***, P < 0.001).