Fig. 5.

Western blotting analysis of the accumulation of Bamboo mosaic virus (BaMV) coat protein in NbGSTU4-GFP or its mutant expressed N. benthamiana plants. (a) The plants were inoculated with BaMV virion and followed by transiently expressed GFP, NbGSTU4-GFP or NbGSTU4/S13A-GFP at 2 d post-inoculation (dpi). The total proteins were extracted at 5 dpi. The protein accumulation levels were measured by western blotting with the antibody against BaMV coat protein or GFP. (b) The accumulation levels of BaMV coat protein were shown as the histogram indicated. The numbers shown above the statistic bar were the average ± SE of at least three independent experiments. Asterisks indicate statistically significant differences between the indicated group analyzed by Student's t-test (***, P < 0.001).