Fig. 7.

The effects of different redox agents in the in vitro RdRp assay. (a) Approximately 50 ng of RNA template r138/40A was incubated with Bamboo mosaic virus (BaMV) RdRp complex for in vitro RNA synthesis in the presence of 50 mM of different redox agents as indicated. (b) The relative RdRp template activities were plotted according to the data derived from (a). The banding density of the in vitro RdRp assay in the presence of 50 mM Tris buffer (pH 7.0) was set as 100%. The numbers shown above the statistic bar were the average ± SE of three independent experiments. Asterisks indicate statistically significant differences between the indicated group analyzed by Student's t-test (**, P < 0.01; ***, P < 0.001).