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. 2013 Apr 25;14(7):810–822. doi: 10.1111/tra.12071

Figure 1. Kainate activation retains KARs in spines.

Figure 1

A) Representative glow-scale images of FRAP timeline for SEP-GluK2 in a dendritic spine. The spine indicated by the circle in the second image was photobleached. To confirm that the FRAP was from surface-expressed SEP-GluK2 we briefly washed cells in pH 6.0 buffer to eclipse the surface of fluorescence. Scale bar 2 µm. B) Normalized traces of SEP-GluK2 FRAP in spines in control (black circles) and kainate (10 μM, 3 min, white circles)-treated neurons. Cells were bleached immediately after kainate treatment (t = 0 min). Mean ± SEM. Left, histograms showing SEP-GluK2 recovery in control and after kainate application. Recovery was defined as the mean value of the steady-state level (400–800 seconds). C) Fitted curves and histograms showing the diffusion coefficients in spines under control and kainate conditions. For (B) and (C) the data are the mean ± SEM, n = 25–36 spines, p < 0.001. D) Left panel, colocalization of endogenous GluK2 (red) and PSD95 (green). Right panel, colocalization of the NR1 (red) and PSD95 (green). Scale bar 2 µm, p < 0.001. Histograms show Pearson's coefficient for the colocalization ± SEM.