Fig. 3.

Effect of overexpression and down-regulation of Pol ι, Pol η or Pol κ on I-SceI-induced homologous recombination. Frequencies of recombination were measured from the frequency of HR-dependent restoration of GFP-positive cells after (a) the overexpression of Pol ι, Pol η or Pol κ; (b) siRNAs knockdown of Pol ι, Pol η, Pol κ; or knockdown of both Pol η and Pol κ. Error bars show standard deviation of the data obtained from three independent experiments. The expression level of the expressed proteins is indicated below panel (a).