Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep;12(9):761–773. doi: 10.1016/j.dnarep.2013.07.001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 The Authors

Open Access under CC BY-NC-SA 3.0 license

PMC Copyright notice

Fig. 6 — DNA binding by F1 + F2 promotes interaction with Ubc9. (A) ^GSTUbc9 does not bind to a 15 bp duplex DNA immobilised on streptavidin agarose via a biotin linkage. Unlabelled DNA is used as a negative control. (B) Under the same conditions, PARP-1 F1 + F2 binds to the 15 bp duplex. (C) PARP-1 F1 + F2 is not significantly retained by SUMO-2 agarose in the presence or absence of DNA. Underivatised agarose is used as a negative control. (D) ^GSTUbc9 binds to SUMO-2 agarose in the absence of DNA. (E) PARP-1 F1 + F2 binds to ^GSTUbc9 immobilised on glutathione Sepharose in the presence, but not in the absence of DNA. GST is used as a negative control. (F) SUMO-1 binds to immobilised ^GSTUbc9 in the absence of DNA.