Figure 4 A.

Co-immunoprecipitation of C-terminal actin with ubiquitin. Density of ubiquitin bands measured between 25-50 kDa representing carboxy-terminal fragments (14 kDa) with bound Ub (multiples of 8kDa, as fragments can be polyubiqiutinated). Fragments above 50 kDa were not measured as may represent ubiquitinated whole actin. Samples represent Pre-HD, End-HD, Control, Control + irrelevant ab (to demonstrate antibody bands at 25 and 50 kDa, and specificity of bands) and western blot of non-immunoprecipitated control representing all Ub.