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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: Free Radic Biol Med. 2012 Jun 23;53(6):1298–1307. doi: 10.1016/j.freeradbiomed.2012.06.024

Fig. 5. Accumulation of lutein and zeaxanthin in A2E- containing RPE cells attenuated blue light induced down-regulation of CFH.

Fig. 5

Confluent ARPE-19 cells that were loaded with A2E alone or loaded with A2E and then incubated with 10 μM lutein or zeaxanthin were exposed to blue light for 10 min. The cells were then cultured in medium containing 1% FBS for another 6 h and the media were collected and cells were harvested. Cells that were neither loaded with A2E nor exposed to blue light were used controls. Levels of mRNA for CFH in the cells were determined by real-time quantitative RT-PCR using levels of mRNA for GAPDH as a reference (panel A) and protein levels of CFH in the medium were determined by ELISA (panel B). The relative levels of mRNA for CFH in control cells were arbitrarily designated as 1 and relative levels of mRNA for CFH in treated cells were expressed as fold of that in the control cells. The data are mean ± SD of the results from 6 samples in each group. * indicates p<0.05 and ** indicates p<0.01 as compared to the control cells that were neither treated with A2E nor blue light.