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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: Free Radic Biol Med. 2012 Jun 23;53(6):1298–1307. doi: 10.1016/j.freeradbiomed.2012.06.024

Fig. 6. Inhibition of the proteasome in RPE cells down-regulates CFH expression.

Fig. 6

Confluent cultured ARPE-19 cells were incubated in fresh medium in the absence or presence of 10 μM MG132 or 5 μM epoxomicin for 8 h. Levels of mRNA for CFH in the cells were determined by real-time quantitative RT-PCR using levels of mRNA for GAPDH as a reference (panel A) and protein levels of CFH in the medium were determined by ELISA (panel B). The relative levels of mRNA for CFH in control cells were arbitrarily designated as 1 and relative levels of mRNA for CFH in MG132- or epoxomicin-treated cells were expressed as fold of that in the control cells. The data are mean ± SD of the results from 4 samples in each group. * indicates p<0.05 and ** indicates p<0.001 as compared to the controls.