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. Author manuscript; available in PMC: 2014 Jul 11.
Published in final edited form as: Mol Cell. 2013 May 30;51(1):35–45. doi: 10.1016/j.molcel.2013.04.021

Figure 1. eIF5A Stimulates Translation of Polyproline Motifs in vivo.

Figure 1

(A) Schematic of Renilla-firefly luciferase reporter construct. Codon repeats were inserted in-frame between the Renilla and firefly luciferase ORFs (Letzring et al., 2010).

(B) Dual luciferase reporter constructs containing 10 repeats of the indicated codon were introduced into isogenic yeast strains expressing wild type eIF5A or temperature-sensitive eIF5A-S149P. (Top panel) Following growth at semi-permissive 33°C, the firefly-to-Renilla luciferase ratio for each construct was normalized to the ratio obtained from reporters with no insert between the ORFs. (Bottom panel) The fold difference in luciferase ratios between cells expressing wild-type eIF5A and eIF5A-S149P was quantitated and then normalized to the values obtained from the no insert control. *Statistical significance for ProCCA(10) was measured by student’s t-test with a p-value <0.05. Error bars were calculated as propagated standard deviations (SD) for three independent transformants.