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. Author manuscript; available in PMC: 2014 Jul 11.
Published in final edited form as: Mol Cell. 2013 May 30;51(1):35–45. doi: 10.1016/j.molcel.2013.04.021

Figure 5. eIF5A Prevents Ribosome Stalling on Consecutive Proline Codons.

Figure 5

Reconstituted peptide synthesis assays were performed in the absence or presence of eIF5A using mRNAs encoding the peptides MFFFFF (left panel) or MPPPPP (right panel). The position of the 80S ribosome was determined by reverse transcription of the mRNA template using a [32P]-labeled primer, and C and T sequencing reactions were run alongside. Reactions lacking elongation factors were performed to identify 80S initiation complexes (IC) on the AUG codon (lanes 3 and 8). The identity of the 80S toeprint signals is indicated on the right; and the sequences of the mRNA and the corresponding amino acids are shown on the left with the sites of ribosome stalls at the 2nd and 3rd proline codons boxed.