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| Tris (mM) | pH | urea (M) | DTT (mM) | mannitol (%) | Pefabloc (μM) | Detergent (BS) (%) | |
|---|---|---|---|---|---|---|---|
| denaturing buffer | 10 | 11 | 6 | 100 | 0.1 | 1 | 5 |
| folding buffer 1 | 10 | 11 | 2 | 0.1 | 0.1 | 0.1 | 0.5 |
| folding buffer 2 | 10 | 11 | 1 | 0.1 | 0.1 | 0.1 | 0.1 |
| folding buffer 3 | 10 | 11 | 0.1 | 0.1 | 0.1 | 0.02 | |
| folding buffer 4 | 10 | 8.8 | 0.1 | 0.1 | 0.1 | ||
| folding buffer 5 | 10 | 8.8 | 0.1 | 0.1 |
