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. Author manuscript; available in PMC: 2013 Aug 16.
Published in final edited form as: Mol Microbiol. 2007 Feb;63(4):1078–1095. doi: 10.1111/j.1365-2958.2006.05582.x

Table 2.

Quantitative analysis of lysoGPCho metabolites in TbPLA1 mutants.a

lysoGPCho seriesb
Cell line Tet (−/18:y) (−/20:y) (−/22:y) Total
WT 53 31 72 156
Δ pla1 15 8 7 30
ovexPLA1-myc − 56 22 81 159
+ 159 42 231 432
Δ plal rescPLAI-myc − 29 14 23 66
+ 52 22 54 128
a

Total lipid extracts from 108 PCF T. brucei cells spiked with 500 pmol of each internal standard [lysoGPCho(17:0/−) and lysoGPCho(24:0/−)] were examined by ESI-MS-MS. lysoGPCho molecules were detected from precursor ion scanning for m/z 184 (see insets from Fig. 5B), and they were quantified by comparison with the internal standards. Values are from a typical analysis and are presented as pmol per 108 cells. Tet, tetracycline.

b

Integration of peaks to obtain peak areas was performed by grouping lysoGPCho into various series of peaks whereby one series comprises both the major and minor lysoGPCho molecules, and their isotopes, containing the same FA chain length but with varying degrees of unsaturation, represented by ‘y’.