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. 2013 Aug 16;5(3):e00119. doi: 10.1042/AN20130010

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Primary human astrocytes. (B) Primary HBMEC; all cells were stimulated with B. burgdorferi for 6–72 h. RNA was extracted and cDNA synthesized. Individual primer sets (SABiosciences) were used to amplify transcripts of interest by QPCR. Data represent at least two biological replicates per time point, with each PCR reaction run in triplicate. Expression levels of all transcripts were compared with housekeeping genes and the relative changes in gene expression were compared with those of untreated cells using the 2^−ΔΔCT method, and are expressed as fold change compared with no spirochete control. Error bars represent S.E.M.