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. 2013 Aug 16;5(3):e00119. doi: 10.1042/AN20130010

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Primary human astrocytes were stimulated with B. burgdorferi for 6, 12, 24, or 72 h. Supernatants from stimulated cells were collected, aliquoted and stored at −80°C. Levels of CXCL-1 (A), CXCL-10 (B), and IL-8 (C) were measured by individual ELISA according to the manufacturer's protocol (R&D Systems). Data represent two biological replicates per time point, with each sample run in triplicate. Error bars represent S.E.M.