Table 3. Transcriptional profiling of chemokine and cytokine gene expression in HBMEC in response to B. burgdorferi.

HBMEC responses to B. burgdorferi. At least two biological replicates were performed per time point. Values shown correspond to the mean ratio of triplicate measurements determined between normalized gene intensity values after 6-72 h of stimulation with 40:1 B. burgdorferi strain B31 compared with gene intensity values from unstimulated cells. Only genes that demonstrated at least a 3-fold change in all biological replicates are included.

(a) 6 h post-stimulation
Gene symbol	Fold regulation	Description/function
CXCL-1	8.6	Chemokine, neutrophil chemoattractant
CXCL-2	14.7	Chemokine
CXCL-3	11.4	Chemokine
IL-8	3.2	Chemokine, neutrophil chemoattractant
TNFα	4.0	Cytokine
(b) 12 h post-stimulation
Gene symbol	Fold regulation	Description/function
CXCL-2	24.9	Chemokine
CXCL-6	11.3	Chemokine
IL-8	22.6	Chemokine, neutrophil chemoattractant
(c) 72 h post-stimulation
Gene symbol	Fold regulation	Description/function
CXCL-6	95.8	Chemokine; T-cell chemotaxis
CXCL-10	327.0	Chemokine; monocyte, NK, T-cell migration