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Effect of addition of SDH (10−6 mg/ml) at different time intervals (10 min before, 10 and 20 min after nitrite) on the time course of Hb oxidation in erythrocyte lysate.
| Time-course for addition of 10−6 mg/ml silibinin | % Formation of MetHb | % Inhibition of MetHb | Time to form 50% MetHb (t1/2) (min) |
|---|---|---|---|
| Control | 100 | 0 | 25 |
| Incubation before 10 min | 17 | 83 | 146 |
| Addition after 10 min | 22.6 | 77.4 | 110 |
| Addition after 20 min | 24.7 | 75.3 | 101 |
Values represent mean of three experiments.
