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Cross-reactions between anti-DNAJ(Hsp40) polyclonal antibodies and DNAJ(Hsp40) proteins tested by Western blotting. The DnaJ, N-DnaJ (DnaJΔ107-375), C-DnaJ (DnaJΔ1-199), DNAJA1, and DNAJA2 recombinant proteins were overproduced in E. coli cells, purified, and resolved by SDS-PAGE. Of each Hsp40 protein, 2.5 μg has been loaded onto the gel (a). The polyclonal antibodies indicated above the panels were used in Western blotting as the primary antibodies (b–f)
