Fig. 2.

Cross-reactions between anti-DNAJ(Hsp40) polyclonal antibodies and DNAJ(Hsp40) proteins tested by ELISA. The reactivity of polyclonal anti-DnaJ (a), anti-N-DnaJ (b), anti-C-DnaJ (c), anti-DNAJA1 (d), and anti-DNAJA2 (e) with purified non-denatured DNAJ antigens was tested as described in “Materials and methods.” Data obtained for the antigens at 40 μg per milliliter are presented as the mean ± SEM of three independent experiments and expressed as a percentage of control (i.e., reactivity with the antigen used to raise a given antibody; indicated as the gray bar)