Fig. 3.

Reactivity of anti-DnaJ monoclonal antibodies (mAbs) with human DNAJA proteins. Schematic outline of the DnaJ linear structure and localization of the epitopes recognized by the mAbs are presented in a. The reactivity of six mAbs (b–g) with purified non-denatured DNAJ(Hsp40) antigens was assayed by ELISA test as described in “Materials and methods.” Data obtained for the antigens at 40 μg per milliliter are presented as the mean ± SEM of three independent experiments and expressed as a percentage of the reactivity with DnaJ control (gray bar)